Chapter 2 JANUARY.

Visit to the ponds-Conferv?-Spirogyra quinina-Vorticella-Common Rotifer-Three divisions of Infusoria-Phytozoa-Protozoa-Rotifera-Tardigrada-Meaning of these terms-Euglen?-Distinction between animals and vegetables-Description of Vorticell?-Dark ground illumination-Modes of producing it-The Nucleus of the Vorticell?-Methods of reproduction-Ciliated Protozoa-Wheel bearers or Rotifers-Their structure-The common Rotifer-The young Rotifer seen inside the old one-An internal nursery-"Differentiation" and "Specialisation"-Bisexuality of Rotifers-Their zoological position-Diversities in their appearanc

e-Structure of their Gizzard-Description of Rotifers.

HE winter months are on the whole less favorable to the collection of microscopic objects from ponds and streams than the warmer portions of the year; but the difference is rather in abundance than in variety, and with a very moderate amount of trouble, representatives of the principal classes can always be obtained.

On a clear January morning, when the air was keen, but no ice had yet skinned over the surface of the water, a visit to some small ponds in an open field not far from Kentish Town provided entertainment for several days. The ponds were selected from their open airy situation, the general clearness of their water, and the abundance of vegetation with which they were adorned. Near the margin conferv? abounded, their tangled masses of hair-like filaments often matted together, almost with the closeness of a felted texture. At intervals, minute bubbles of air, with occasionally a few of greater size, indicated that the complex processes of vegetable life were actively going on, that the tiny plants were decomposing carbonic acid, dexterously combining the carbon-which we are most familiar with in the black opaque form of charcoal-to form the substance of their delicate translucent tissues, and sending forth the oxygen as their contribution to the purification of the adjacent water, and the renovation of our atmospheric air. This was a good sign, for healthy vegetation is favorable to many of the most interesting forms of infusorial life. Accordingly the end of a walking-stick was inserted among the green threads, and a skein of them drawn up, dank, dripping, and clinging together in a pasty-looking mass. To hold up a morsel of this mass, and tell some one not in the secrets of pond-lore that its dripping threads were objects of beauty, surpassing human productions, in brilliant colour and elegant form, would provoke laughter, and suggest the notion that you were poking fun at them, when you poked out your stick with the slimy treasure at its end. But let us put the green stuff into a bottle, with some water from its native haunt, cork it up tight, and carry it away for quiet examination under the microscope at home.

Here we are with the apparatus ready. We have transferred a few threads of the conferva from the bottle to the live box, spreading out the fine fibres with a needle, and adding a drop of water. The cover is then gently pressed down, and the whole placed on the stage of the microscope, to be examined with a power of about sixty. A light is thrown somewhat obliquely by the mirror through the object, the focus adjusted, and a beautiful sight rewards the pains. Our mass of conferva turns out to contain one of the most elegant species. Fine hair-like tubes of an organic material, as transparent as glass, are divided by partitions of the same substance into cylindrical cells, through which a slender ribbon of emerald green, spangled at intervals with small round expansions, is spirally wound. We shall call it the Spiral Conferva, its scientific name being Spirogyra quinina. Some other species, though less elegantly adorned, make a pleasing variety in the microscopic scene; and appended to some of the threads is a group of small crystal bells, which jerk up and down upon spirally twisted stalks. These are the "Bell Flower Animalcules" of old observers, the Vorticell?, or Little Vortex-makers of the present day. Other small creatures flit about with lively motions, and among them we observe a number of green spindles that continually change their shape, while an odd-looking thing crawls about, after the manner of certain caterpillars, by bringing his head and tail together, shoving himself on a step, and then repeating the process, and making another move. He has a kind of snout, behind which are two little red eyes, and something like a pig-tail sticks out behind. This is the Common Wheel-bearer, Rotifer vulgaris, a favourite object with microscopists, old and young, and capable, as we shall see, of doing something more interesting than taking the crawl we have described.

A higher power, say one or two hundred, may be conveniently applied to bring out the details of the inhabitants of our live box more completely; but if the glasses are good, a linear magnification of sixty will show a great deal, with the advantage of a large field, and less trouble in following the moving objects of our search.

Having commenced our microscopic proceedings by obtaining some Euglen?, Vorticell?, and a Rotifer, we are in a position to consider the chief characteristics of three great divisions of infusoria, which will often engage our attention.

It is well known that animalcules and other small forms of being may be found in infusions of hay or other vegetable matter, and hence all such and similar objects were called Infusoria by early observers. Many groups have been separated from the general mass comprehended under this term, and it is now used in various senses. The authors of the 'Micrographic Dictionary' employ it to designate "a class of microscopic animals not furnished with either vessels or nerves, but exhibiting internal spherical cavities, motion effected by means of cilia, or variable processes formed of the substance of the body, true legs being absent." The objection to this definition is, that it to some extent represents theories which may not be true. That nerves are absent all through the class is an assumption founded merely upon the negative evidence of their not having been discovered, and the complete absence of "vessels" cannot be affirmed.

In the last edition of 'Pritchard's Infusoria,' to which some of our ablest naturalists have contributed, after separating two groups, the Desmids, and the Diatoms, as belonging to the vegetable world, the remainder of the original family of infusoria are classified as Phytozoa, Protozoa, Rotifera, and Tardigrada. We shall explain these hard names immediately, first remarking that the Desmids and the Diatoms, concerning whom we do not intend to speak in these pages, are the names of two groups, one distinctly vegetable, while the other, although now generally considered so, were formerly held by many authorities to be in reality animal. The Desmids occur very commonly in fresh water. We have some among our Conferv?. They are most brilliant green, and often take forms of a more angular and crystalline character than are exhibited by higher plants. The Diatoms are still more common, and we see before us in our water-drop some of their simplest representatives in the form of minute boats made of silica (flint) and moved by means still in dispute.

Leaving out the Desmids and Diatoms, we have said that in Pritchard's arrangement the views of those writers are adopted who divide the rest of the infusoria into four groups, distinguished with foreign long-tailed names, which we will translate and expound. First come the Phytozoa, under which we recognise our old acquaintance zoophyte turned upside down. Zoophytes mean animal-plants, Phytozoa mean plant-animals. We shall have by-and-bye to speak of some of the members of this artificial and unsatisfactory group, and postpone to that time a learned disquisition on the difference between animals and plants, a difference observable enough if we compare a hippopotamus with a cabbage, but which "grows small by degrees, and beautifully less," as we contemplate lower forms.

After the Phytozoa come the Protozoa, or first forms in which animality is distinctly recognised. Under this term are assembled creatures of very various organization, from the extreme simplicity of the Proteus or Am?ba, a little lump of jelly, that moves by thrusting out portions of its body, so as to make a sort of extempore legs, and in which no organs can be discerned,[2] up to others that are highly developed, like our Vorticell?. This group is evidently provisional, and jumbles together objects that may be widely separated when their true structure and real affinities are discerned.

[2] In some kinds and in some stages of growth this is not strictly true.

Following the Protozoa, come the Rotifera, or Wheel-bearers, of which we have obtained an example from our pond, and whose characteristics we shall endeavour to delineate when our specimen is under view; and last in the list we have the Tardigrada, "Slow-steppers," or Water Bears, queer little creatures, something like new-born puppies, with a double allowance of imperfect feet. These, though somewhat connected with the rotifers, are considered to belong to a low division of the arachnida (spiders, &c.).

a, motile; and b, resting condition of Euglen?.

Feeling that we must be merciful with the long-tailed words and explanations of classification, we reserve further matter of this kind for the opportunities that must arise, and direct our attention to living forms by watching the Euglen? which our water-drop contains. We have before us a number of elegant spindle-shaped bodies, somewhat thicker in front than behind, and in what may be called the head there glitters a brilliant red speck, commonly called an eye-spot, although, like the eyes of potatoes, it cannot see. Round this eye-spot the tissues are clear, like glass; but the body of the creature is of a rich vegetable green, which shines and glistens as it catches the light. Some swim rapidly with a rollicking motion, while others twist themselves into all manner of shapes. Now the once delicate spindle is oddly contorted, now it swells out in the middle, like a top, and now it rolls itself into a ball. The drawings will afford some idea of these protean changes, but they must be seen before their harlequin character can be thoroughly appreciated. Some of the specimens exhibit delicate lines running lengthwise, and taking a spiral twist as the creature moves about; but in none can any mouth be discerned, and their antics, although energetic and comical, afford no certain indications of either purpose or will. What are they? animals or vegetables? or something betwixt and between?

The first impression of any casual observer would be to declare in favour of their animality; but before this can be settled, comes the question, what is an animal, and how does it differ from a vegetable? and upon this the learned do by no means agree. One writer considers the presence of starch in any object a proof that it belongs to the dominions of Flora, while another would decide the issue by ascertaining whether it evolves oxygen and absorbs carbon, as most plants do, or whether it evolves carbon and absorbs oxygen, as decided animals do. Dr. Carpenter asserts that the distinction between Protophyta and Protozoa (first or simplest plants and animals), "lies in the nature of their food, and the method of its introduction, for whilst the Protophyte obtains the materials of its nutrition from the air and moisture that surround it, and possesses the power of detaching oxygen, hydrogen, carbon, and nitrogen from their previous binary combinations, and of uniting them into ternary and quaternary organic compounds (chlorophyll, starch, albumen, &c.), the simplest Protozoa, in common with the highest members of the animal kingdom, seems utterly destitute of any such power, makes, so to speak, a stomach for itself in the substance of its body, into which it injects the solid particles that constitute its food, and within which it subjects them to a regular process of digestion."

Unfortunately it is very difficult to apply this simple theory to the dubious objects which lie on the border-land of the animal world, and no other theory that has been propounded appears to meet all cases. Some naturalists do not expect to find a broad line of demarkation between the two great divisions of living things, but others characterise such an idea as "unphilosophical," in spite of which, however, we incline towards it.

Mr. Gosse, whose opinion is entitled to great respect, calls the Euglen? "animals" in his 'Evenings with the Microscope;' but from the aggregate of recorded observations it seems that they evolve oxygen, are coloured with the colouring matter of plants, reproduce their species in a manner analogous to plants, and have in some cases been clearly traced to the vegetable world. It is, however, possible that some Euglen? forms may be animal and others vegetable, and while their place at nature's table is being decided, they must be content to be called Phytozoa, which, as we have before explained, is merely Zoophyte turned upside down.

Some authorities have thought their animality proved by the high degree of contractility which their tissues evince. This, however, cannot go for much, as all physiologists admit contractility to belong to the vegetable tissues of the sensitive plant, "Venus' Fly-trap," &c., and a little more or less cannot mark the boundary between two orders of being.

We shall have occasion again to notice the Protophytes, and now pass to the Protozoa, of which we have a good illustration in the Vorticella already spoken of. In the group before us a number of elegant bells or vases stand at the end of long stalks, as shown at the top of the frontispiece, while round the tops of the bells, the vibrations of a wreath or cilia produce little vortices or whirlpools, and hence comes the family name. This current brings particles of all sorts to the mouth near the rim of the bells, and the creature seems not entirely destitute of power to choose or reject the morsels according to its taste. Every now and then the stalk of some specimen is suddenly twisted into a spiral, and contracted, so as to bring the bell almost to the ground. Then the stem gracefully elongates again, and the cilia repeat their lively game.

The general effect can be seen very well by a power of about sixty linear, but one of them from one to two hundred is necessary to bring out the details, and a practised observer will use still more magnification with good effect. They should be examined by a moderately oblique light, or most of the cilia are apt to be rendered invisible, and also by dark ground illumination. This may be accomplished in a well-made microscope by turning the mirror quite out of the plane of the axis of the instrument, that is to say, on one side of the space the body would occupy if it were prolonged. By this means, and by placing the lamp at an angle with the mirror, that must be learnt by experiment, all the light that reaches the eye has first passed through the object, and is refracted by it out of the line it was taking, which would have carried it entirely away. Or the object may be illuminated by an apparatus called a spotted lens, which is a small bull's-eye placed under the stage, and having all the centre of its face covered with a plaster of black silk. In this method the central or direct rays from the mirror are obstructed, but those which strike the edge of the bull's-eye are bent towards the object, which they penetrate and illuminate if it is sufficiently transparent and refractive. Another mode of dark ground illumination is by employing an elegant instrument called a parabolic illuminator, which need not be described.

Left: Vorticella, with posterior circlet of cilia in process of separation-Stein.

Right: Vorticella in process of self-division. A new frontal wreath in formation in each of the semi-lunar spaces.

Different specimens and species of Vorticell? vary in the length of their bells from one three or four thousandth to one hundred and twentieth of an inch, and when they are tolerably large, the dark ground illumination produces a beautiful effect. The bells shine with a pearly iridescent lustre, and their cilia flash with brilliant prismatic colours.

Left: Vorticella microstoma, showing alimentary tube, ciliated mouth, and formation of a gemma at the base, 300 linear.-Stein.

Right: Vorticella microstoma, the encysted animal protruding through a supposed rupture of the tunic.

The Vorticellina belong to the upper division of the Protozoa-the ciliata, or ciliated animalcules, and they have a mouth, an ?sophagus, and an orifice for the exit of their food.

Many observers used to ascribe to those creatures a complete intestinal canal, but such an apparatus is now believed not to exist in any of the Infusoria. Food particles, after leaving the ?sophagus, are thrust forward into the sarcode, or soft flesh, and any cavity thus formed acts as a stomach.

The bells or cups are not, as might be fancied from a casual inspection, open like wineglasses at the top, but furnished with a retractile disk or cover, on which the cilia are arranged. Their stalks are not simple stems, but are hollow tubes, which in the genus Vorticella are furnished with a muscular band, by whose agency the movements are principally made.

Some of the Vorticellids will be observed to leave their stalks, having developed cilia round their base, and may be seen to swim about in the enjoyment of individual life. They are also capable of becoming encysted, that is, of secreting a gelatinous cover.

Encysted Vorticella, showing the obliteration of special organs by the advancement of the process.-Pritchard.

These changes are exhibited in the annexed cuts, which are copied from known authorities. By careful observation of the bodies of Vorticellids, a contractile vesicle may be observed, which appears to cause a movement of fluids, that is probably connected either with respiration or secretion.

Another piece of apparatus in this family, but not confined to it, is the so-called nucleus, which in this case is of a horseshoe shape and granular texture, and greater solidity than the surrounding parts. The functions of this organ formed the subject of various conjectures, but it is now generally held to be an ovary.

Vorticella microstoma, in process of encystment, 300 linear; in the last the inclosing tunic is plainly developed.-Stein.

In common with many of the lower animals, the Vorticellids have three ways of multiplying their race. One by fission, or division of their bodies: another by buds, somewhat analogous to those of plants; and another by reproductive germs. These processes will come again under our notice, and we shall leave the Vorticellids for the present by observing that if they are fed with a very small quantity of indigo or carmine, the vacuoles or spaces, into which their nutriment passes, will be clearly observed. Ehrenberg thought in these and similar creatures that every vacuole was a distinct stomach, and that all the stomachs were connected by an intestinal canal; hence his name Polygastrica, or many stomached. In these views he has not been followed by later observers, and it is probable he was misled, partly by pushing the process of reasoning from the analogies of higher animals much too far, and partly by the imperfection of the glasses he employed.

Rotifer vulgaris.-A, mouth, or gizzard; B, contractile vesicle.-Micrographic Dictionary.

N.B.-When the cilia and tail part are retracted, and the body shortened, the creature assumes an obtuse oval form.

Having thus briefly considered the Vorticellids we must turn to the wheel-bearer, who belongs to a higher race than even the ciliated Protozoa. We left her crawling about with her snout or proboscis protruded, but now she has moored herself by her tail-foot, pulled in her nose, and put out two groups of cilia, which look like revolving wheels, and a little below them is seen a gizzard in a state of active work. After a little while she swims away with her wheels going, and her tail, forked at the end, is found to be telescopic, or capable of being pulled in and out. As the cilia play, the neighbouring water is agitated, and the multitudes of small objects are brought by the whirlpools within her ravenous maw. But the strangest thing of all is that inside her body is seen a young one; in this case a large and fine infant, which, like "a chip of the old block," imitates the parental motions, thrusts forth its cilia and works its gizzard.[3] In other genera the eggs are hatched externally, but this one is ovoviparous, and carries its nursery inside.

[3] This was met with in the summer, but is described here to avoid repetition. I do not know whether the eggs are hatched in very cold weather.

A very slight investigation is sufficient to show that in the wheel-bearer we have made a great advance towards a higher organization than we discovered in the preceding creatures. We witness what the learned call a "differentiation" of parts and tissues, and a "specialization" of organs. The head is plainly distinguishable from the body, the skin or integument is distinctly different from the internal tissues, behind the eyes we can detect a nervous ganglion or miniature brain, the gizzard is a complicated piece of vital mechanism, such as we have not met with before, and in various parts of the transparent inside we see organs to which particular functions are assigned.

It was at one time thought that Rotifers were hermaphrodite-uniting both sexes in one body-but that idea is now generally abandoned, for in many species the males have been discovered, and the fair sex may be gratified to hear that they are without doubt the "inferior animals." Their function is simply to assist the female in producing young, and as this can be quickly accomplished, their lives are short, and they are not supplied with the gizzard and digestive apparatus, which their lady-loves possess. Much discussion has taken place as to the rank which the Rotifers hold in the animal kingdom, some naturalists thinking them relations of the crabs, and others believing them to belong to the family of the worms. Professor Huxley, who adopts the latter view, which has the most friends, groups the lower Annulosa together under the name of Annuloida, in which he includes Annelides, or worms of various kinds, the Echinodermata (or "spine skins," among which are the star-fish and sea hedgehogs), and some other families. He considers the Rotifers to be "the permanent forms of Echinoderm larv?." This does not mean that they were ever produced by Echinoderms, and had their development checked, but that they resemble them in organization, and illustrate a general law, observable in animated beings, namely, that the lower creatures are like the imperfect stages of higher animals, and that all things are formed according to general principles, and exhibit a uniformity of plan.

Mr. Gosse adopts a different view, and while admitting a connection between the Rotifers and the worms, adduces important reasons for associating them with the insects.

Leaving zoologists to settle their position, we may remark that the Rotifers form a very numerous family, presenting very great diversities of structure, some of the most interesting of which we shall meet with in the course of our rambles; but they all possess a gizzard, which, though differing in complexity, is throughout formed upon the same principle, and that we must now explain.

We have called the masticatory apparatus of the Rotifers a gizzard; but Mr. Gosse, who has done most to elucidate its structure, contends that it is a mouth; and in some species it is frequently protruded, and used like the mouth of higher animals. Taking one of the most typical forms of this organ, and drawing our illustrations from Mr. Gosse's admirable paper in the "Transactions of the Royal Society," we may describe it, when completely developed, as consisting of three lobes, having a more or less rounded form. The eminent naturalist we have named calls the whole organ the mastax, and states that it is composed of dense muscular fibre. The tube which leads down to it he designates the "buccal (mouth) funnel," and the tube that issues from it, and conveys the food to the digestive sac or stomach, he calls the ?sophagus, in conformity with the nomenclature applied to creatures whose mouths are in the usual place. Inside the mouth-gizzard are placed two organs, which work like hammers, and which Mr. Gosse therefore names mallei. The hammers work against a sort of anvil, which is called incus, the Latin for that implement. Each hammer consists of two portions articulated by a hinge joint. The lower portion, the manubrium, or handle, gives motion to the upper portion, which from its shape is named the uncus, or hook. The unci are furnished with finger-like processes of teeth, which vary in number. There are five or six in the best developed specimens. These hooks or teeth work against each other, and against the incus, or anvil, which consists of distinct articulated portions, of which the principal are two rami, or branches, jointed so that they can open and close like a pair of shears. These two rest upon the third portion, which is called the fulcrum. Some faint idea of the working of the toothed hammers may be obtained by rubbing the knuckles of both hands together, but the motion is more complicated, and the rami play their part in the trituration of the food. Mr. Gosse states that when an objectionable morsel has got as far as this mouth-gizzard, "it is thrown back by a peculiar scoop-like action of the unci, very curious to witness." The foregoing diagram will help the reader to comprehend this description, but no opportunity should be lost for viewing this remarkable organ busy at work in the living animals.

Gizzard of Notomata.

The respiration of the Rotifers is supposed to be effected by the passage of water through vessels running round them, and called the "water vascular system," and in addition to their eyes, which often disappear in adult specimens, the organ we described as standing out like a pig-tail, as our acquaintance crawled along, is thought to act as an antenna, or feeler, and brings its possessor in further relation to the external world. It is also called the calcar, or spur, and is furnished with cilia or bristles at its extremity.

Sometimes the particles swallowed by the Common Rotifer are large enough for their course to be traced, but there is frequently a great commotion and grinding of the gizzard, without any appreciable cause, although doubtless something is taken in, and when the creature is tired, or has had enough, we see both head and tail retracted, and the body assumes a globular form. In another chapter, when viewing a Philodine, we shall see how in the family to which the Common Rotifer belongs, the gizzard departs from the perfect type.

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